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KMID : 0043319970200010068
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Archives of Pharmacal Research
1997 Volume.20 No. 1 p.68 ~ p.71
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Optimal Immobilization of Penicillinase for Ion-selective Electrode
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Hur Moon-Hye
Kang Hee-Jin
Min Hye-Young
Lee Ji-Yeun
Lee Ki-Hyun
Ahn Moon-Kyu
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Abstract
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Penicillin sensor was prepared by immobilizing penicillinase (Pcase) on -selective carboxylated poly (vinyl chloride) (PVC-COOH) membrane or cellulose filter membrane. The immobilization techniques are as follows. Pcase was immobilized with GTH on -selective PVC-COOH membrane or some amount of BSA was dropped on that membrane. Another method to make immobilization is to mix type I Pcase with GTH and drop on a cellulose filter membrane. According to immobilization techniques, there were some differences in response properties of enzyme electrodes, however, all electrodes responded to Pcase-resistant penicillin derivatives. Pcase immobilized on cellulose filter membrane with -selective PVC membrane eletrode was more stable and more sensitive to penicillinase-resistant penicillin derivatives than any other immobilization techniques.
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KEYWORD
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Immobilization, Penicillinnase, H[+]-selective membrance, Cellulose filter membrance
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